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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Cancer Res. 2016 Apr 13;76(11):3211–3223. doi: 10.1158/0008-5472.CAN-15-0025

Figure 3. Conditional deletion of p53 abrogates the treatment response to xRT.

Figure 3

A,B) IHC for p53 demonstrates accumulation of p53 protein 2 hours after xRT in M-Smo tumors with intact p53. C,D) In M-Smo;p53floxed tumors, p53 is absent in tumor cells but detectable in adjacent stroma and in endothelial cells within the tumors. E–J) In situ hybridization demonstrates mRNA for Puma and p21 as indicated. Both Puma and p21 mRNAs are induced by xRT in M-Smo tumors. Post-xRT expression of Puma and p21 is reduced in tumors with conditional deletion of p53. K–M) Cleavage of caspase 3 is not induced in M-Smo;p53floxed tumors by xRT at either 4 hours, 24 hours or 5 days after treatment. N–P) IHC for PH3 demonstrates mitotic figures. M-Smo;p53floxed tumors demonstrate absence of mitosis 4 hours after xRT, but demonstrate frequent mitoses 24 hours and 5 days after treatment. Scale bars: 1mm (A,C and K–P), 100 µm (B,D and E–J), and 50 µm (insets).