Figure 6.

Loss of p120 catenin induces significant transcriptional changes in genes affecting S1P/S1pr2 signaling. A) qPCR showed significant downregulation of genes involved in the biosynthesis of S1P in KC^iMist1; p120^f/f pancreata (n=3 pancreata) when compared to KC^iMist1; p120^wt/wt pancreata (n=4 pancreata). B,C) Western blot images and quantification shown are representative of 3 independent experiments. D) IF images show effective p120 catenin knockdown in siRNA treated CFPAC-1 spheres. E) Western blot depicts expression of p120 catenin and S1pr2 in CFPAC-1 cells. F) Quantification shown represents n=3 experiments for each condition. Scale bars are 50μm.