Fig.2.

Changes in hepatic mRNA expression of pro-inflammatory cytokines (A) Tnfα, (B) Il6, (C) Cxcl2, and (D) Cd14 in saline-treated male mice receiving EtOH (n=10) and EtOH/SPI (n=10) diets compared to chow (n=5) controls. Western blot analysis of (E) nuclear expression of NFκB expression liver tissue from EtOH and EtOH/SPI fed mice. Data are expressed as mean ± St. Err. For real-time RT-PCR, statistical analysis was determined by Kruskal-Wallis One-way Analysis of Variance on Ranks followed by Dunn's post-hoc analysis, and groups with different letters are significantly different from each other. For the Western, statistical significance was determined by Student's T-test. Means that do not share a letter are significantly different at p<0.05