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. 2016 Jun 3;7:849. doi: 10.3389/fmicb.2016.00849

FIGURE 4.

FIGURE 4

GIE492 excision frequency increased upon addition of mitomycin C to the culture medium or overexpressing the island-encoded integrase. Excision frequency was measured by qPCR starting from genomic DNA isolated from KpRYC492 cells cultured until different phases of growth in a medium without or supplemented with mitomycin C (mito C). (A) Growth curves of KpRYC492 in LB medium supplemented with up to 2 μg/ml mito C. Arrows indicate the points from which genomic DNA was extracted. (B) Dose-dependent mito C-mediated increase of GIE492 excision frequency. (C) Effect of overexpressing the island-encoded integrase over the excision frequency. KpRYC492 cells were transformed with either pint (allowing IPTG-inducible expression of int gene) or pCA24N (control) and grown in LB supplemented with IPTG, or IPTG plus mito-C until the indicated phase of growth. Error bars correspond to the standard deviation of two measurements from three independent experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.