FIGURE 5.

Genes from GIE492 encoded in the MccE492-production cluster and genes unrelated to this bacteriocin production are transcribed at comparable levels. Relative abundances of GIE492-encoded transcripts were measured by qRT-PCR, starting from total RNA isolated from KpRYC492 cells cultured until mid-exponential or late-exponential phase of growth. Expression values were calculated considering the amplification efficiencies of each primer pair used to amplify every target gene. rpoD gene was used to normalize. Error bars indicate the standard deviation among four measurements from two independent experiments.