FIGURE 6.
asn-tDNA loci as integration hotspots for genomic islands in Klebsiella pneumoniae. (A) A total of 47 GIs (colored squares) integrated in any of the four asn-tRNA loci (asn1A to asn1D) were identified among the assembled chromosomes of 52 K. pneumoniae strains. Those GIs can be classified into 12 homology groups (see color code). (B) Schematic representation of the main features of each asn1-GI group. Blue rectangles and red arrows represent direct repeats and integrase-coding genes, respectively. Black and green arrowheads represent insertion sequences and transfer origins (oriT), respectively. 1Previously described as GI-I (Lery et al., 2014); 2Previously described as ICEKp1 (Lin et al., 2008). R–M, restriction–modification; HPs, hypothetical proteins of unknown function; MT, methyltransferase; NA, nucleic acids.