Figure 3.

Tracheal allografts manifest HMGB1 passive release and active secretion along with altered HPSE expression. A. Cold ischemic insult (4 hours) resulted in HMGB1 passive release as indicated by arrows within the inset picture. B. Detection of HMGB1 passive release from tracheal allografts (indicated by arrows within the inset picture) after 24 hours of transplantation. C. Co-immunostaining for analysis of HMGB1 active secretion by allograft infiltrated macrophages after day 14 of transplantation. HMGB1 stained in green, macrophages stained by F4/80 in red, and nucleus stained in blue by DAPI. D. Tracheal allografts with BOS onset were manifested by active HPSE expression. E. Results for co-immunostaining of α-SMA and HPSE in tracheal grafts at day 28 of transplantation. Active HPSE was stained green, α-SMA was stained in red, and nucleus was stained in blue.