Figure 4.

mRNA and protein expressionof MI-HF SD rats. A-C: Effect of QL in mRNA expression in CHF rats. The mRNA level were determined by RT-PCR and normalized to GAPDH housekeeping gene. Values are mean ± SD, *P<0.05 vs. N group; #P<0.05 vs. V group. D, E: QL inhibits mitochondrial-mediated apoptosis in HF rats caused by chronic MI. D: Representative western blot analysis demonstrating the expression of Bcl-2 and Bax in different groups. Densitometric analysis: E: In HF rats caused by MI, the ratio of Bcl-2/Bax was reduced and QL treatment increased the Bcl-2/Bax ratio conversely. F: QL reduced the expression levels of cleaved caspase-3 caused by HF. Data are analyzed by one-way ANOVA followed by the Tukey post-hoc and expressed as the mean ± SD, *P<0.05 vs. V group.