Figure 2.

The expressions of CREPT were significantly inhibited in cells transfected with CREPT-siRNA. A. Micrograph of Calu-1 and H838 cells infected with CREPT-siRNA for three days in bright and fluorescent fields (400× magnification). More than 80% of cells expressed GFP. B. RT-PCR results showing the CREPT mRNA levels were inhibited in cells transfected with CREPT-siRNA as compared with NC-siRNA and blank control. C. Western blot analysis showing the expression levels of CREPT protein in cells transfected with CREPT-siRNA was remarkable lower than that of NC-siRNA and blank control cells. * indicates P<0.05.