Figure 1.

The schematic of the experiment. NOD/SCID mice were subcutaneously injected with Caco-2 cells to form tumor. Then orthotopic implantation of an identical size of tumor of 1 mm diameter was performed in NOD/SCID mice to create CRC. After tumor implantation, the mice received GRh2 at different doses for 4 weeks. The CRC-mice that received saline only were used as controls (Control). Serum cytokine levels were measured at the end of the experiment and the mice were subjected to 3 different behavior tests to evaluate the levels of depression. The mice were then kept to evaluate the lifespan without further treatment.