Figure 1.

Isolation of bone marrow-derived mesenchymal stem cells and differentiation into tri-lineages. (A) Fibroblast-like cells at 2 weeks post seeding; scale bar = 500 μm (B) Osteogenic, adipogenic, and chondrogenic differentiation of isolated BM-MSCs was confirmed by intracellular alkaline phosphatase (ALP) activity (C; n = 9 for each), intracellular accumulation of lipid droplets (D), and the concentration of acidic mucopolysaccharide (E; n = 5 for each), respectively. Scale bar = 100 μm. *p < 0.01.