Figure 4.

Sesamin promoted adenosine production and upregulated the expression of CD39 and A2AR in the kidney after ischemic reperfusion. C57/BL6 mice (n = 6) were pretreated with vehicle or sesamin (100 mg/kg) every 8 h one time for three times within 24 h before surgery. Twenty-four hours after reperfusion, kidney tissues were collected for next analysis. A. The concentration of adenosine in the kidney was measured by HPLC. B. The mRNA expression of CD39, CD73 and A2AR in the kidney was evaluated by qRT-PCR. C. The protein expression of CD39, CD73 and A2AR in the kidney was detected by western blotting. Data are expressed as the mean ± SD. *P<0.05; **P<0.01.