Figure 2.

HOTTIP overexpression promoted cell growth and cell cycle by activating Wnt/ β-catenin pathway. (A) qRT-PCR revealed that HOTTIP was efficiently overexpression by transfected with pcDNA3.1-HOTTIP in MG63 cells. (B, C) Overexpression of HOTTIP significantly promoted cell proliferation (B) and enhanced colony formation (C) compared to the negative control (NC). (D) Overexpression of HOTTIP significantly increased the expression levels of cyclin D1, CDK4 and β-catenin in MG63 cells. (E). Overexpression of HOTTIP led an increase in the number of cells in the S phase and a decrease in the percentage of cells in the G0/G1 phase, *P<0.05.