Figure 3.

HOTTIP knockdown suppressed cell growth and cell cycle by inhibiting Wnt/β-catenin pathway. (A) qRT-PCR suggested that HOTTIP was efficiently downregulation by transfected with si-HOTTIP in MG63 cells. (B, C) Decreased expression of HOTTIP significantly inhibited cell proliferation (B) and reduced colony formation (C) compared to the negative control (NC). (D) Decreased expression of HOTTIP significantly decreased the expression levels of cyclin D1, CDK4 and β-catenin in MG63 cells. (E) Reduced expression of HOTTIP led a decrease in the number of cells in the S phase and an increase in the percentage of cells in the G0/G1 phase. (F) In MG63 cells, higher HOTTIP expression predicted cellular resistance to cisplatin, *P<0.05.