Figure 3.

Western blot assays of cell signaling proteins. While total levels of P13K, AKT, MEK, and ERK showed no changes, the levels of phospho-P13K, phospho-AKT, phospho-MEK, and phospho-ERK were observed to be significantly lower in wild-type parafibromin expressing cells. Untreated: OS cells without treatment; Recombinant: OS cells treated with recombinant parafibromin; Wild-type: OS cells were transfected with pEGFP-C2-parafibromin plasmid; Mutagenesis: site directed mutagenesis was carried out on pEGFP-C2-parafibromin. IGF-1 was used as a positive control.