Figure 3.

The LGR5 splice variants were not expressed during cell cycle arrest.(A) Expression of LGR5 mRNA among LPC. Lovo expressed high levels of LGR5 mRNA. (B) Schematic illustration of the starve test. (C) qRT–PCR of the mRNA extracted from collected Lovo shows that the expression of CDKN1A decreased gradually after the peak at 3 h, whereas LGR5 mRNA slowly increased after serum addition, peaking after 12 h. (D) As a result of electrophoresis of the PCR-purified products, multiple bands were revealed after 3 and 12 h.