FIGURE 6.

Th17 cell maintenance is not required for EAE immunopathology. (A) Flow cytometry for IFN-γ and IL-17 from naive T cells from Rag1^WT and Rag1^ΔRORa mouse lines polarized in vitro toward Th1 or Th17 cells. (B) Clinical EAE scores of Rag1^WT and Rag1^ΔRORa mouse lines immunized with MOG/CFA (n = 6–7). (C) Clinical EAE scores of IL-17A^WT and IL-17A^ΔRORγt mouse lines immunized with MOG/CFA (n = 8/group). (D) Numbers of total CD4⁺ T cells, RFP⁺ Th17 lineage-positive, and RFP⁻ Th17 lineage-negative cells present in the CNS of IL-17A^WT controls (white bars) or IL-17A^ΔRORγt mice (gray bars) upon EAE induction at day 17 (averages ± SEM., n = 6). (E and F) Flow cytometry for indicated cytokines on RFP⁺ Th17 lineage-positive (E) or RFP⁻ lineage-negative (F) cells obtained from IL-17A^WT and IL-17A^ΔRORγt mice undergoing EAE, as shown in (C). (G) Numeric presence of total CD4⁺ T cells expressing indicated cytokines in the CNS of IL-17A^WT controls (white bars) or IL-17A^ΔRORγt (gray bars) during EAE at day 17 (averages ± SEM, n = 6). *p < 0.05, **p < 0.01, ***p < 0.001.