Table 2.

Selected candidate reference genes, primers, and amplicon characteristics.

Gene	Primer sequences (forward/reverse)	Amplicon length (bp)	Tm (°C)	Amplification efficiency
CYP	AAGGAGATGGAGAAGGTTGGTT	213	83.37	2.072
	CCCTTTCTGACTTTGGGTAGACATA
RPL	TCAGACAGTGATTACACCGAGTTC	246	80.87	1.914
	GCCAGTAGAGACAAAAAGGCAAGA
GAPDH	GTCGCTTGGCTGCCTATAATCT	204	81.79	1.962
	AGACCGTTGACTGAACCATCC
TUA	GATTATGAGGAAGTTGGGGCTG	175	81.09	1.986
	AAGCAACCTAGCACATAGTGAAAGT
TUB	AGATGTTCCGTCGTGTGAGTGA	86	83.43	1.977
	CCTCCTCATATTCATCCTCATCAG
Fe-SOD	AAGAGGAGAAAGAGCAAGAGTCAGA	114	80.80	1.917
	CCGATACAACAAACCCTGAAATG
Mn-SOD	ACTACCTACAGTACAAGAATGTCAGACC	184	81.44	1.949
	GGGCTCCTCATCCTATATCGTT
Cu/Zn-SOD	TTGTAGGAAGGGCTGTCGTTG	207	82.46	1.992
	TCTCGTCAAGTCACTCTCAAGCAT
18SrRNA	CCTGAGAAACGGCTACCACAT	171	83.93	1.931
	CACCAGACTTGCCCTCCA
Actin	TGCTATCCTTCGGTTGGACC	93	81.77	1.984
	CGGACGATTTCCCGTTCAG
Histone H3	ATCCGCAAGTACCAGAAGAGCA	155	85.71	2.002
	CCCACCAAGTAAGCCTCAG
EF-1a	GATGATTCCCACCAAGCCCAT	129	84.57	2.072
	GGGTCCTTCT TCTCAACACTCT