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. 2016 Jun 3;6:27177. doi: 10.1038/srep27177

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Copyright © 2016, Macmillan Publishers Limited

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(a) PYLs interact with MYB44 in the yeast two-hybrid assay. PYLs fused to the GAL4-DNA-binding domain (BD) were used as bait. MYB44 fused to the GAL4-activating domain (AD) were used as preys. Interaction was determined by yeast growth on media lacking His, Leu and Trp. BD-PYL10 and AD-ABI1 was used as a positive control. (b) PYL9 interacts with MYBs in the yeast two-hybrid assay. PYL8 and MYB combinations were used as positive controls and PYL6 and MYBs were used as negative controls. Interaction was determined by growth on medium lacking His, Leu and Trp with or without 5 μM ABA. Dilutions (10⁻¹, 10⁻², and 10⁻³) of saturated cultures were spotted onto the plates, which were photographed after 5 days. (c) Co-transform of PYL9-nLUC with MYB44-cLUC, MYB73-cLUC, MYB77-cLUC and MYB61-cLUC in Col-0 wild-type protoplasts in LUC complementation assay.