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. 2016 Jun 3;6:27126. doi: 10.1038/srep27126

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Copyright © 2016, Macmillan Publishers Limited

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(a) CK cells were infected with Beau-R (open circles) or M41 (closed circles) at an MOI of 0.005. Release of progeny virus was determined by plaque assay. Mean and standard error of three independent experiments are shown. (b) Genomic RNA levels were measured by two-step RT-qPCR at 8 and 24 hours after inoculation of CK cells with 20 pfu per cell of Beau-R or M41. Results from three independent experiments are shown. Non-significant differences by t-test are indicated (ns). CK cells were inoculated with (c) Beau-R for 4 hours or (d) M41 for 3 hours. Cells were fixed with 4% paraformaldehyde and labelled with anti-dsRNA (green) and nuclei were labelled with TO-PRO-3 (red).