Table 2. Quantitation of markers of infection in cells infected with different IBV strains.
Virus | Cell | Type | Total Cells | Visibly infected cell sections | Per visibly infected cell section | Infected cell sections | Infected cell sections with one or more | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
DMV | Spherule | IV | Any | DMV | Spherule | IV | DMV | Spherule | IV | ||||||||
H120 | TOC 24 hpi | V-AP | n = 210 | 7% | 6% | 9% | 11% | 4 ± 1 | 4 ± 1 | 15 ± 5 | n = 24 | 63% | – | 54% | – | 79% | – |
Beau-R | L-AP | n = 63 | 29% | 17% | 46% | 52% | 3 ± 1 | 4 ± 1 | 18 ± 4 | n = 69 | 58% | ns | 54% | ns | 78% | ns | |
M41 | L-P | n = 395 | 8% | 1% | 12% | 13% | 2 ± 1 | 0.1 ± 0.1 | 19 ± 4 | n = 53 | 62% | ns | 6% | p = 4 × 10−6 | 92% | ns | |
D1466 | F-P | n = 157 | 9% | 3% | 13% | 13% | 2 ± 1 | 1 ± 1 | 27 ± 5 | n = 21 | 67% | ns | 24% | ns | 95% | ns | |
4/91 | F-P | n = 79 | 15% | 10% | 18% | 23% | 2 ± 1 | 2 ± 1 | 11 ± 4 | n = 21 | 67% | ns | 48% | ns | 76% | ns | |
Italy02 | F-P | n = 60 | 10% | 5% | 10% | 18% | 3 ± 1 | 3 ± 2 | 17 ± 5 | n = 16 | 50% | ns | 31% | ns | 63% | ns | |
Beau-R | CK cell 24 hpi | L-AP | n = 76 | 14% | 14% | 33% | 37% | 2 ± 1 | 3 ± 1 | 35 ± 11 | n = 52 | 42% | – | 40% | – | 90% | – |
M41 | L-P | n = 84 | 19% | 2% | 37% | 39% | 1 ± 0.3 | 0.3 ± 0.2 | 21 ± 4 | n = 34 | 50% | ns | 9% | p = 1 × 10−3 | 94% | ns |
V = vaccine, L = lab adapted, F = field, AP = apathogenic, P = pathogenic. The intracellular abundance of virus-induced features in individual cell-sections was compared to H120 using Fisher’s exact test.