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. 2016 Jun 3;6:27169. doi: 10.1038/srep27169

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Copyright © 2016, Macmillan Publishers Limited

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The strains SC-19, ΔrelA, ΔrelQ and ΔrelAΔrelQ were grown in the MOPS-CDM containing 150 μCi/ml H₃[³²P]O₄, and 1.0% or 0.2% glucose at 37 °C for 30 min. The formic acid extracts of the cells were subjected to TLC analysis as described in Materials and Methods.