| Pan-IAV assay |
| IAV-M1.2 |
Hoffmann et al.15
|
|
| IAV-M1-F |
AGA TGA GTC TTC TAA CCG AGG TCG |
20 μM |
| IAV-M1.1-R |
TGC AAA AAC ATC TTC AAG TYT CTG |
15 μM |
| IAV-M1.2-R |
TGC AAA GAC ACT TTC CAG TCT CTG |
15 μM |
| IAV-M1-FAM |
FAM-TCA GGC CCC CTC AAA GCC GA-BHQ1 |
2.5 μM |
| H1 assays |
| IAV-H1-Mix 3-FAM |
(this study) |
|
| IAV-H1-115F |
ACA CAA TAT GTA TAG GYT AHC ATG C |
20 μM |
| IAV-H1-199R |
GAG TGT GTY ACT GTY ACA TTC TT |
20 μM |
| IAV-H1-147FAM |
FAM-TCD ACM GAC ACT GTW GAC ACA GTA CTN GA-BHQ1 |
5 μM |
| IAV-H1-Mix 27-FAM |
(this study) |
|
| IAV-H1-1078F |
AGG AAT GTC CCR TCY ATT CAA TC |
20 μM |
| IAV-H1-1086F |
CCC GTC YAT TCA ATC YAG AGG |
20 μM |
| IAV-H1-1180R |
GGT GAT AAC CRT ACC ANC CAT C |
20 μM |
| IAV-H1-1190R |
TCA TTT TGA TGR TGA TAA CCR TAC CA |
20 μM |
| IAV-H1-1155.1FAM |
FAM-CAT YCC WGT CCA YCC YCC TTC AAT GAA-BHQ1 |
5 μM |
| H2 assays |
| IAV-H2-Mix 4-FAM |
(this study) |
|
| IAV-H2-470-F |
GAC ACA GCA YAC RAC AAC TGG |
15 μM |
| IAV-H2-601-R |
GTG TTG TTG TAT GAT CYT TTR GCA A |
15 μM |
| IAV-H2-522-FAM |
FAM-CCN TCA TTC TTC AGG AAC ATG GTY TGG-BHQ1 |
5 μM |
| IAV-H2-Mix 10-FAM |
(this study) |
|
| IAV-H2-1075-F |
CAA GRG GAT TGT TTG GRG CAA T |
15 μM |
| IAV-H2-1175-R |
TGA TCC YTG RTC ATT GCT GTG |
15 μM |
| IAV-H2-1142-FAM |
FAM-CCA RCC ATC AAC CAT YCC TTG CCA TCC-BHQ1 |
5 μM |
| H3 assays |
| IAV-H3-Mix 3-FAM |
(this study) |
|
| IAV-H3-876-F |
CTC CTC GGG GTT AYT TYA AAA T |
15 μM |
| IAV-H3-975-R |
CCA TTT GGA GTG ATR CAT TCA GA |
15 μM |
| IAV-H3-934-FAM |
FAM-TGC ATC TGA YCT CAT TAT TGA GCT TTT CCC-BHQ1 |
5 μM |
| IAV-H3-Mix 14-FAM |
(this study) |
|
| IAV-H3-1667-F |
TGG ATT TCC TTT GCC ATA TCA TG |
15 μM |
| IAV-H3-1784-R |
ATR CAC TCA AAT GCA AAT GTT GCA |
15 μM |
| IAV-H3-1753-FAM |
FAM-CTA ATG TTG CCT YTY TGG CAG GCC CAC AT-BHQ1 |
5 μM |
| H4 assay |
| IAV-H4-Mix 15-FAM |
(this study) |
|
| IAV-H4-1586F |
GAC TCA RGG ATA CAA RGA CAT |
15 μM |
| IAV-H4-1599F |
AAG GAC ATC ATY CTY TGG ATT TC |
15 μM |
| IAV-H4-1686R |
ACA AGC CCA CAA AAT RAA GGC |
15 μM |
| IAV-H4-1696R |
TTC CRT TYT GAC AAG CCC ACA A |
15 μM |
| IAV-H4-1628FAM |
FAM-TCC ATA TCA TGC TTY TTR CTC GTT GC-BHQ1 |
5 μM |
| H5 assays |
| IAV-H5-Mix 1-FAM |
modified Spackman et al.25 CRL Weybridge |
|
| IAV-H5-1F |
ACA TAT GAC TAC CCA CAR TAT TCA G |
25 μM |
| IAV-H5-1R |
AGA CCA GCT AYC ATG ATT GC |
25 μM |
| IAV-H5-1FAM |
Fam-TCW ACA GTG GCG AGT TCC CTA GCA-BHQ1 |
5 μM |
| IAV-H5a-Mix 1-FAM |
(this study) |
|
| IAV-H5a-1658F |
GTT CCC TAG YAC TGG CAA TCA T |
20 μM |
| IAV-H5a-1747R |
AAT TCT ARA TGC AAA TTC TGC AYT G |
20 μM |
| IAV-H5a-1685FAM |
FAM-CTG GTC TAT CYT THT GGA TGT GYT CCA ATG-BHQ1 |
5 μM |
| H6 assay |
| IAV-H6-Mix 8-FAM-MGB |
(this study) |
|
| IAV-H6-1666F |
CTT GGT GTG TAT CAA ATY CTT GC |
20 μM |
| IAV-H6-1776R |
CAT TGA RCC ATT TGA RCA CAT CCA |
20 μM |
| IAV-H6-1693FAM-MGB |
FAM-TAT AGT ACG GTA TCG AGC AGY CT-MGB |
5 μM |
| H7 assays |
| FLI-H7generic-2 |
Kalthoff et al.23
|
|
| IAV-HA7-1593-F |
AYA GAA TAC AGA TWG ACC CAG T |
20 μM |
| IAV-HA7-1740-R |
TAG TGC ACY GCA TGT TTC CA |
20 μM |
| IAV-HA7-1649-FAM |
FAM-TGG TTT AGC TTC GGG GCA TCA TG –BHQ1 |
2.5 μM |
| IAV-H7-2.2-Mix-FAM |
(this study) |
|
| IAV-HA7-1617-F |
AAA TTG AGC AGY GGM TAC AAR GA |
25 μM |
| IAV-HA7-1707-R |
AAA ACC ART CCC ATT RCA ATG GC |
25 μM |
| IAV-HA7-1649.1-FAM |
FAM-TGG TTT AGC TTC GGG GCR TCA TGY TT_BHQ1 |
5 μM |
| FLI-H7-CODA |
van Borm et al.26
|
|
| IAV-HA7-CODA-F |
GYA GYG GYT ACA AAG ATG TG |
20 μM |
| IAV-HA7-CODA-R |
GAA GAC AAG GCC CAT TGC AA |
20 μM |
| IAV-HA7-CODA-FAM |
FAM-TGG TTT AGC TTC GGG GCA TCA TG-BHQ1 |
2.5 μM |
| H8 assay |
| IAV-H8-Mix 1-FAM |
(this study) |
|
| IAV-H8-1604F |
TAC AAA ATT CTY AGC ATY TAC AGT AC |
20 μM |
| IAV-H8-1677R |
ATT ARA CCT CCA GCA AYC AGG A |
20 μM |
| IAV-H8-1654FAM |
FAM-TGC CAA GCA RAG ACT GGC CGC CA-BHQ1 |
5 μM |
| H9 assay |
| IAV-H9-Mix 2-FAM |
modified Monne et al.24
|
|
| IAV-H9-2F |
ATG GGG TTT GCT GCC |
20 μM |
| IAV-H9-2R |
TTA TAT ACA AAT GTT GCA YCTG |
20 μM |
| IAV-H9-2FAM |
FAM-TTC TGG GCC ATG TCC AAT GG-BHQ1 |
5 μM |
| H10 assays |
| IAV-H10-Mix 5-FAM |
(this study) |
|
| IAV-H10-991F |
GTT GCT TGC WAC MGG AAT GAG |
15 μM |
| IAV-H10-1041F |
GCC TGT TTG GDG CRA TAG C |
15 μM |
| IAV-H10-1122R |
TTT TGR TGT CKG AAR CCA TAC CA |
15 μM |
| IAV-H10-1173R |
ATA GCT GCY TGA GTA CTY TTG TA |
15 μM |
| IAV-H10-1092FAM |
FAM-ACC ATY CCT TCC CAT CCR TTY TCT A-BHQ1 |
6.25 μM |
| IAV-H10-Mix 9-FAM |
(this study) |
|
| IAV-H10-1567F |
GAA ACT CTC TTC TGG VTA YAA AGA |
20 μM |
| IAV-H10-1695R |
ATT GTG CAT CGC ATG TTT CCA T |
20 μM |
| IAV-H10-1601FAM |
FAM-TGG TTT AGC TTC GGG GCR TCA TGY TT-BHQ1 |
5 μM |
| H11 assay |
| IAV-H11-Mix 1-FAM |
(this study) |
|
| IAV-H11-1510F |
ARG TYA GGA ATG GAA CAT ATG AYC A |
20 μM |
| IAV-H11-1723R |
CAA ATG GTA CAT CTA CAT GAY CCA |
20 μM |
| IAV-H11-1626FAM |
FAM-ATT TAC AGC TGC ATY GCA AGY AGT CT_BHQ1 |
12.5 μM |
| H12 assay |
| IAV-H12-Mix 3-FAM |
(this study) |
|
| IAV-H12-1607F |
AGC ATC TAC AGC AGT GTY GC |
20 μM |
| IAV-H12-1707R |
CAG AAA GTA CAA CGA AYA TTT CCA |
20 μM |
| IAV-H12-1674FAM |
FAM-CCG AAA ATG AAA CCC CCA ATA ATC ATG A-BHQ1 |
5 μM |
| H13 assay |
| IAV-H13-Mix 2-FAM |
(this study) |
|
| IAV-H13-1241F |
ATT GAC AAA ATG AAT GGR AAY TAT GAY TC |
20 μM |
| IAV-H13-1372R |
AAG AAG YTT DGC ATT RTA TGA CCA |
20 μM |
| IAV-H13-1304FAM |
FAM-ATA AAY ATG CTY GCA GAY AGR ATA GAT GAY GC-BHQ1 |
5 μM |
| H16 assay |
| IAV-H16-Mix 6-FAM-MGB |
(this study) |
|
| IAV-H16-1589F |
GGG ATA AAR TTG AAR ACT GAR GA |
20 μM |
| IAV-H16-1708R |
ACT GCT RCA TGC CCA CAK TAT |
20 μM |
| IAV-H16-1635FAM-MGB |
FAM-TTT AYA GYT GCA TTG CAA GCA G-MGB |
5 μM |
| N1 assay |
| IAV-N1-Mix 3-FAM |
(this study) |
|
| IAV-N1-3-F |
AGR CCT TGY TTC TGG GTT GA |
25 μM |
| IAV-N1-3-R |
ACC GTC TGG CCA AGA CCA |
25 μM |
| IAV-N1-3-FAM |
FAM-ATY TGG ACY AGT GGG AGC AGC AT-BHQ1 |
5 μM |
| N2 assay |
| IAV-N2-Mix 5.5 FAM-MGB |
(this study) |
|
| IAV-N2-1367F |
AGT CTG GTG GAC YTC AAA YAG |
30 μM |
| IAV-N2-1488R |
AAT TGC GAA AGC TTA TAT AGV CAT |
30 μM |
| IAV-N2-1444.1FAM-MGB |
FAM-CCA TCA GGC CAT GAG CCT-MGB |
5 μM |
| N3 assay |
| IAV-N3-Mix 2-FAM |
(this study) |
|
| IAV-N3-1348F |
AAY AGT ATA GTT ACT TTC TGY GG |
20 μM |
| IAV-N3-1422R |
CCA ATG TTR GAA CCA TCH GG |
20 μM |
| IAV-N3-1373FAM |
FAM-TAR ACA ATG AAC CTG GAT CGG GVA A-BHQ1 |
5 μM |
| IAV-N3-Mix 4-FAM |
(this study) |
|
| IAV-N3-1348F |
AAY AGT ATA GTT ACT TTC TGY GG |
20 μM |
| IAV-N3-1422R |
CCA ATG TTR GAA CCA TCH GG |
20 μM |
| IAV-N3-1440R |
TDT TAC TTG GGC ATD AAC CCA AT |
20 μM |
| IAV-N3-1373FAM |
FAM-TAR ACA ATG AAC CTG GAT CGG GVA A-BHQ1 |
5 μM |
| N4 assay |
| IAV-N4-Mix 6-FAM |
(this study) |
|
| IAV-N4-1335F |
GAC YAG TGG TAG TAG YAT YGC |
20 μM |
| IAV-N4-1345F |
AGT AGY ATT GCR TTY TGT GGT GTT |
20 μM |
| IAV-N4-1437R |
AAA TYA CTT GTC TAT GTC AAA DGG |
20 μM |
| IAV-N4-1387FAM |
FAM-TGG TCR TGG CCY GAT GGC GCT CT-BHQ1 |
5 μM |
| N5 assay |
| IAV-N5-Mix 5-FAM |
(this study) |
|
| IAV-N5-1322F |
AAG AGA GRA CWA GCA TTT GGA C |
15 μM |
| IAV-N5-1353F |
CTC CAC KGT RTT TTG TGG TGT |
15 μM |
| IAV-N5-1421R |
GGA AGA ATT GCK CCA TCA YC |
15 μM |
| IAV-N5-1426R |
CAA AKG GAA GAA TTG CKC CAT CA |
15 μM |
| IAV-N5-1375FAM |
FAM-TCM AGT GAG GTC CCA GGR TGG TC-BHQ1 |
5 μM |
| N6 assay |
| IAV-N6-Mix 3-FAM |
(this study) |
|
| IAV-N6-10F |
AGG GTG AAR ATG AAT CCA AAY CA |
20 μM |
| IAV-N6-14F |
TGA ARA TGA ATC CAA ATC AGA AGA TAA |
20 μM |
| IAV-N6-97R |
CAA TCC TAT YAG CAG RCT TAC TAC |
20 μM |
| IAV-N6-43FAM |
FAM-TGC ATH TCA GCH ACA GGA ATG ACA CTA TC-BHQ1 |
5 μM |
| N7 assay |
| IAV-N7-Mix 1-FAM |
(this study) |
|
| IAV-N7-1305F |
GTT GAA TTA ATW AGA GGA AGR CC |
20 μM |
| IAV-N7-1430R |
GAT YTG TGC CCC ATC RGG GA |
20 μM |
| IAV-N7-1383FAM |
FAM-AGC CCA DTC YCA GTT GGG TCY GGT TC-BHQ1 |
5 μM |
| N8 assay |
| IAV-N8-Mix 1-FAM |
(this study) |
|
| IAV-N8-1296F |
TCC ATG YTT TTG GGT TGA RAT GAT |
20 μM |
| IAV-N8-1423R |
GCT CCA TCR TGC CAY GAC CA |
20 μM |
| IAV-N8-1354FAM |
FAM-TCH AGY AGC TCC ATT GTR ATG TGT GGA GT-BHQ1 |
10 μM |
| N9 assay |
| IAV-N9-Mix 11-FAM |
Kalthoff et al.23
|
|
| IAV-N9-1363F |
AGY ATA GTA TCR ATG TGT TCC AG |
20 μM |
| IAV-N9-1439R |
AAG TAC TCT ATT TTA GCC CCA TC |
20 μM |
| IAV-N9-1393FAM |
FAM-TTC CTB GGA CAA TGG AAC TGG CC-BHQ1 |
5 μM |
| IC assay |
| EGFP-Mix2- HEX |
Hoffmann et al.16
|
|
| EGFP-1-F |
GAC CAC TAC CAG CAG AAC AC |
5 μM |
| EGFP-10-R |
CTT GTA CAG CTC GTC CAT GC |
5 μM |
| EGFP-HEX |
HEX-AGC ACC CAG TCC GCC CTG AGC A-BHQ1 |
3.75 μM |
