Table 4. Reactivity of HA subtype-specific RT-qPCR assays used in RITA with the validation panel of 404 viral isolates.
| RT-qPCR | H1 | H2 | H3 | H4 | H5 | H6 | H7 | H8 | H9 | H10 | H11 | H12 | H13 | H14 | H15 | H16 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| IAV-H1-Mix 3 | 51 | – | – | – | – | – | – | – | – | – | – | – | - | – | – | – |
| IAV–H1–Mix 27 | 53 | – | – | – | – | 37* | – | 3* | – | – | – | – | – | – | – | – |
| IAV–H2–Mix 4 | – | 18 | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| IAV–H2–Mix 10 | – | 18 | – | – | 1* | - | – | – | – | – | – | – | – | – | - | – |
| IAV–H3–Mix 3 | – | – | 35 | – | – | – | – | – | – | – | – | – | – | – | – | – |
| IAV–H3–Mix 14 | – | – | 40 | – | – | – | – | – | – | – | – | – | – | – | – | – |
| IAV–H4–Mix 15 | – | – | – | 17 | – | – | – | – | – | – | – | – | – | – | – | – |
| IAV-H5-Mix 1 | – | – | – | – | 87 | – | – | – | – | – | – | – | – | – | – | – |
| IAV–H5a–Mix 1 | – | – | – | – | 88 | – | – | – | – | – | – | – | – | – | – | – |
| IAV-H6-Mix 8 | – | – | – | – | – | 61 | – | – | – | – | – | – | – | – | – | – |
| IAV-H7-CODA | – | – | – | – | 1* | – | 33 | – | – | 1* | – | – | – | – | 1* | – |
| IAV–H7-Mix 2 | – | – | – | – | – | – | 35 | – | – | 6* | – | – | –– | – | – | – |
| IAV-H7-Mix 2.2 | – | – | – | – | – | – | 35 | – | – | – | – | – | – | – | – | – |
| IAV-H8-Mix 1 | – | – | – | – | – | – | – | 3 | – | – | – | – | – | – | – | – |
| IAV-H9-Mix 2 | – | – | – | – | – | – | – | – | 31 | – | – | – | – | – | – | – |
| IAV-H10-Mix 5 | – | – | 30# | – | – | – | – | – | – | 29 | – | – | – | – | – | – |
| IAV-H10-Mix 9 | – | – | – | – | – | – | 30† | – | – | 29 | – | – | – | – | – | – |
| IAV-H11-Mix 1 | – | – | – | – | – | – | – | – | – | – | 11 | – | – | – | – | – |
| IAV-H12-Mix 3 | – | – | – | – | – | – | – | – | – | – | – | 1 | – | – | – | – |
| IAV-H13-Mix 2 | – | – | – | – | – | – | – | – | – | – | – | – | 10 | – | – | – |
| IAV-H16-Mix 6 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 4 |
| total | 54 | 18 | 40 | 17 | 88 | 61 | 35 | 3 | 31 | 29 | 11 | 1 | 10 | 1 | 1 | 4 |
*Δ Cq between pan assay and false positive reactivity ≥10 and Δ Cq between specific assay and false positive reactivity ≥8.
#Δ Cq between pan assay and false positive reactivity ≥3 and Δ Cq between specific assay and false positive reactivity ≥3.
†Δ Cq between pan assay and false positive reactivity ≥5; and Δ Cq between specific assay and false positive reactivity ≥4.