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. 2016 Jun 3;6:27174. doi: 10.1038/srep27174

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PANC-1 cells in the presence of different NT4 concentrations (20, 10 and 5 μM) were seeded on the upper component of trans-well inserts of uncoated or collagen IV–coated Boyden chambers and then incubated for 24 h. Migrating cells (A) were counted as described in the Methods section. (B) Representative images of migrated cells after 24 h, fixed and stained with Crystal violet. **p < 0.01, ***p < 0.001 calculated using one-way ANOVA with DUNNETT post-test; n (sample size) = 10.