Fig. 4.

Expression of folate receptors in breast cancer cell lines. The expression of (A) SLC19A1, (B) SLC46A1 and (C) FOLR1 were measured in MCF10A, MCF7 and Hs578T treated with folic acid (100 nmol/l; ■) using quantitative RT-PCR. Values are means, with standard errors represented by vertical bars. Expression levels were normalised to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and are relative to MCF10A cells treated with 0 nmol/l folic acid (□). Data were analysed by two-way ANOVA using Bonferroni's post hoc correction. Mean values with unlike letters were significantly different (P < 0·05).