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. 2016 Jun 3;3(3):ENEURO.0081-16.2016. doi: 10.1523/ENEURO.0081-16.2016

Figure 15.

Figure 15.

Summary of bilateral pre-BötC optical perturbations of inspiratory frequency, the amplitude of inspiratory phrenic activity, and TI and TE in the reduced in situ preparations from VgluT2-Arch-GFP, Dbx1-Arch-GFP, and Sst-tdTomato-Arch-GFP (n = 6 each) Tg lines. A, Laser application significantly reduced inspiratory frequency monotonically in a laser power-dependent manner in all cases. These frequency–power relations were not statistically different among VgluT2-, Dbx1-, and Sst-expressing neuronal populations (one-way ANOVA: F(2,15) = 0.60, p = 0.564). B, TI did not significantly change (F(3,16) = 0.56, p = 0.649). C, TE increased in a laser power-dependent manner, which accounts for the progressive reductions of inspiratory frequency. D, The amplitudes of phrenic inspiratory bursts were not significantly changed (F(3,16) = 0.66, p = 0.589). Data are represented as the mean ± SD.