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. 2016 Jun 3;3(3):ENEURO.0081-16.2016. doi: 10.1523/ENEURO.0081-16.2016

Figure 4.

Figure 4.

Glutamatergic phenotype of VgluT2- and Sst-expressing pre-BötC neurons in the VgluT2-tdTomato-GAD67-GFP triple-transgenic mouse line. A, Confocal images of a subregion within the pre-BötC in a fixed coronal section from the VgluT2-tdTomato-GAD67-GFP triple-Tg strain, showing Cre-dependent VgluT2-tdTomato neuronal labeling (red), GAD67-knock-in GFP neuronal labeling (green), and immunolabeling with glycine antibody (blue) in the pre-BötC neurons. Merged image confirms no overlap of tdTomato neuronal somal labeling (examples indicated by arrows) with GAD67-GFP or glycine antibody expression (arrowheads), except for labeling of terminals in some cases, also verifying that all the Cre-driven tdTomato-labeled VgluT2 neurons are glutamatergic neurons. B, Confocal images of a pre-BötC subregion in an adult VgluT2-tdTomato-GAD67-GFP mouse, illustrating Cre-dependent VgluT2-tdTomato neuronal labeling (red), GAD67-knock-in GFP neuronal labeling (green), immunolabeling with glycine antibody (blue), and Sst antibody labeling (white) of pre-BötC neurons. Merged image illustrates that tdTomato-labeled VgluT2 neurons coexpress Sst (arrows), but GAD67-GFP- or glycine antibody-positive neurons do not express Sst (arrowheads). All images have the same dorsomedial anatomical orientation. d, Dorsal; m, medial.