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. 2016 Jun 3;3(3):ENEURO.0081-16.2016. doi: 10.1523/ENEURO.0081-16.2016

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Copyright © 2016 Koizumi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 5. — Validation of Sst-Cre line and Sst antibody labeling of pre-BötC glutamatergic and Dbx1-derived neurons. A, Confocal fluorescence microscopy images at low magnification showing Sst antibody-labeled neurons within the pre-BötC in a representative fixed coronal section from adult VgluT2-tdTomato mouse. VS, Ventral surface; d, dorsal; m, medial. B, C, Confocal fluorescence microscopy images of a pre-BötC subregion at higher magnification showing VgluT2 Cre-dependent tdTomato neuronal labeling (red; B), Dbx1 Cre-dependent tdTomato labeling (red; C), and immunolabeling with Sst antibody (green) in pre-BötC neurons. Arrows in the merged image in B and C indicate the coexpression of Sst in tdTomato-labeled VgluT2-expressing and Dbx1-derived neurons. D, Confocal images of a pre-BötC subregion in a fixed section from the Sst-tdTomato double-Tg mouse strain illustrating tdTomato neuronal labeling (red) and immunolabeling with Sst antibody (green) colocalized with tdTomato (arrows and merged image). All images have the same dorsomedial anatomical orientation.