Table 1.
Antibody Table: Antibodies for Western Blots, IHC, and ICC
| Name of Antibody | Manufacturer and Catalog Number | Species Raised, Monoclonal or Polyclonal | Dilution Used |
|---|---|---|---|
| E-cadherin | Cell Signaling Technology, 3195 | Rabbit, monoclonal | 1:1000 (W); 1:400 (IHC) |
| ZO1 | Cell Signaling Technology, 8193 | Rabbit, polyclonal | 1:1000 (W) |
| Pan-keratin | Cell Signaling Technology, 4545 | Mouse, monoclonal | 1:1000 (W); 1:500 (IHC) |
| β-Catenin | Cell Signaling Technology, 8480 | Rabbit, monoclonal | 1:1000 (W); 1:100 (IHC, ICC) |
| Glycodelin A (PAEP) | Abcam Inc, ab103264 | Rabbit, polyclonal | 1:1000 (W), 1 μg/mL |
| Cx43 | Cell Signaling Technology, 3512 | Rabbit, polyclonal | 1:1000 (W); 1:200 (IHC); 1:75 (ICC) |
| Connexin 32 | Invitrogen (Thermo Fisher Scientific), 71-0600 | Rabbit, polyclonal | 1:500 (W), 0.5 μg/mL |
| N-cadherin | Cell Signaling Technology, 13116 | Rabbit, monoclonal | 1:1000 (W); 1:125 (IHC) |
| Fibronectin | Abcam Inc, ab2413 | Rabbit, polyclonal | 1:5000 (W); 1:250 (IHC, ICC) |
| CD10 | Abcam Inc, ab79423 | Rabbit, monoclonal | 1:2500 (W), 0.2 μg/mL |
| CD10 | Dako, M7308 | Mouse, monoclonal | 1:50) (IHC) |
| β-Actin | Sigma-Aldrich, A4700 | Mouse, monoclonal | 1:1000 (W) |
| β-Actin | Sigma-Aldrich, A2066 | Rabbit, monoclonal | 1:1000 (W) |
| β-Actin | Cell Signaling Technology, 3700 | Mouse, monoclonal | 1:600 (ICC) |
| TCF8/ZEB1 | Cell Signaling Technology, 3396 | Rabbit, monoclonal | 1:1000 (W) |
| Vimentin | Cell Signaling Technology, 5741 | Rabbit, monoclonal | 1:1000 (W) |
| DDR2 | Cell Signaling Technology, 3396 | Rabbit, polyclonal | 1:1000 (W) |
| Antirabbit IgG (H + L) F(ab′)2 fragment | Cell Signaling Technology, 4412 | Alexa Fluor488 conjugate | 1:250 (ICC) |
| Antimouse IgG (H + L) F(ab′)2 fragment | Cell Signaling Technology, 4409 | Alexa Fluor555 conjugate | 1:250 (ICC) |
| Goat antimouse IgG (H + L) peroxidase-conjugated antibody | Thermo Scientific Pierce, 31430 | Goat | 1:30 000 (W) |
| Goat antirabbit IgG (H + L) peroxidase-conjugated antibody | Thermo Scientific Pierce, 31460 | Goat | 1:30 000 (W) |
Abbreviations: TCF8, transcription factor-like 8; W, Western (blots); ZEB1, zinc finger E-box binding homeobox 1.