Increased serum and adipose RBP4 levels in cardiac hypertrophy. Serum RBP4 levels (A); Rbp4 mRNA expression in WAT, BAT, and liver (B); and serum Ang-II levels (C) in mice with TAC or sham procedures (n = 6 per group). *, P < .05 vs sham. Serum RBP4 levels (D) and Rbp4 mRNA expression (E) in WAT, BAT, and liver of mice with Ang-II or saline infusion (n = 6 per group). *, P < .05 vs saline. F and G, Primary adipocytes were pretreated with Ang-II receptor inhibitor losartan (100 μM for 6 h), followed by stimulation with Ang-II (1 μM for 48 h). F, Rbp4 mRNA levels. G, RBP4 protein levels (n = 6 per group). *, P < .05 vs control; #, P < .05 vs Ang-II alone; AU, arbitrary units.