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RBP4 induces hypertrophic responses in primary cardiomyocytes. A–D, Cardiomyocytes were treated with recombinant mouse RBP4 or vehicle control for 48 hours. Hypertrophy was assessed by cell size (A), cell surface area measurement (B), protein to DNA ratio (C), and analysis of the mRNA expression for Anp, Bnp, and Myh7 by qRT-PCR (D) (n = 6 per group). *, P < .05 vs control; #, P < .05 vs RBP4 with 50 μg/mL. Bar, 20 μm. AU, arbitrary units.
