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. 2016 Apr 21;157(6):2282–2293. doi: 10.1210/en.2015-2022

Figure 4.

Figure 4.

RBP4 enhances oxidative stress in primary cardiomyocytes. A and B, Cardiomyocytes were treated with recombinant mouse RBP4 or vehicle control for 48 hours. Oxidative stress was assessed by mRNA expression of Nox2, Nox4, Nrf2, Nqo1, and Ho1 (A) and intracellular ROS production (B) (n = 6 per group). *, P < .05 vs control; #, P < .05 vs RBP4 with 50 μg/mL. C–F, Cardiomyocytes were pretreated with antioxidant NAC (10 mM for 1), and then recombinant mouse RBP4 was added (50 μg/mL for 48 h). C, mRNA expression of Nox2, Nox4, Nrf2, Nqo1, and Ho1. D. Intracellular ROS production. E, mRNA expression of Tnf-α, Il-6, Mcp-1, and Il-1β. F, mRNA expression of Anp, Bnp, and Myh7 (n = 6 per group). *, P < .05 vs control; #, P < .05 vs RBP4 alone. AU, arbitrary units.