Table 6.
Relative gray value of different enzymes calculated using MUC and CMC zymograms (%)
| Lines | Enzyme | S = 0 | S = 3.6 | S = 3.9 | S = 4.2 | |
|---|---|---|---|---|---|---|
| 2 | EG | Cel5C, Cel5B | 3.73 | 23.97 | 19.05 | 27.87 |
| 3 | Cel5C, Cel7B | 13.45 | 51.62 | 57.73 | 71.02 | |
| 4 | Cel5B | 2.97 | 16.02 | 15.13 | 28.12 | |
| 5 | Cel5B | 47.92 | 66.41 | 73.69 | 80.04 | |
| Sum | 33.61 | 51.6 | 53.12 | 61.41 | ||
| 6 | Xylanase | Xylanase10A | 14.49 | 25.93 | 33.86 | 30.76 |
| 1 | BGL | Bgl1 | 38.75 | 98.42 | 87.91 | 84.55 |
| 4 | CBH | Cel7a-2 | 31.05 | 60.80 | 50.22 | 44.20 |
The enzyme without adsorption by lignin was set as control, and the gray value is the percentage relative to the gray value of the homologous line in the control