Table 1.

Schistocephalus solidus specimens from three different developmental stages used to generate the de novo reference transcriptome^b

Sample ID	Mass (mg)	Stagea	Library size (no. raw reads)	Platform	Ave. read quality (PHRED score)
ssol.cltw.NI.05	1.7	NI	95.7 M	HiSeq 2000	37
ssol.cltw. NI.08	1.5	NI	57.6 M	HiSeq 2000	37
ssol.cltw. NI.12	5.2	NI	29.5 M	HiSeq 2000	37
ssol.cltw. NI.13	1.8	NI	69.6 M	HiSeq 2000	37
ssol.cltw. NI.14	3.4	NI	59.8 M	HiSeq 2000	37
ssol.cltw. NI.22	7.7	NI	49.1 M	HiSeq 2000	37
ssol.cltw. NI.26	13.1	NI	53.7 M	HiSeq 2000	37
ssol.cltw. NI.63	102	I	37.5 M	HiSeq 2000	37
ssol.cltw. NI.67	90.1	I	42.3 M	HiSeq 2000	37
ssol.cltw. I.98–1	101.2	I	36.8 M	HiSeq 2000	37
ssol.cltw.I.98–2	108.1	I	56.3 M	HiSeq 2000	37
ssol.cltw.A.01	164.5	A	38.8 M	MiSeq	38
ssol.cltw.A.03	321	A	61.2 M	HiSeq 2000	37
ssol.cltw.A.07	329.4	A	50.2 M	HiSeq 2000	37
ssol.cltw.A.12	356	A	51.7 M	HiSeq 2000	37

^a NI non-infective, I infective, A adult

^bWorms in bold were selected as the three representative samples to be used to perform the initial raw de novo assembly. All 14 HiSeq libraries were then used to eliminate redundancy in the final dataset and increase assembly quality, while sample cltw.a-01 (in italic) was used to perform preliminary optimization tests on assembly parameters