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. 2016 Jun 3;35:87. doi: 10.1186/s13046-016-0366-3

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Cell migration and invasion assays: The experiments were performed in transwell chambers with non-Matrigel or Matrigel coated membranes by using stable transfected SK-Hep-1 cell lines with pCMV-Tag2A-wX, pCMV-Tag2A-X and control vectors. Cells were stained with crystal violet and photographed (magnification, ×200). Data shown as mean ± SD of each group from three independent experiments. Compared with the control cells, HBx cells showed significantly enhanced migration and invasion capacity (**P < 0.01, *P < 0.05), HBwx also showed a promoting effect on migration and invasion. However, the migratory and invasive ability of HBwx cells was significantly reduced compared with HBx cells (**P < 0.01, *P < 0.05)