Table 3.
List of genes which transcription is enhanced by CBD in TMOG cells (p < 0.005)
| Symbol | Gene name | Accession number | MOG alone [fold]$ | MOG+CBD [fold]$ | Change [%] | CBD alone [fold]$ |
|---|---|---|---|---|---|---|
| MOG35-55-upregulated and CBD-enhanced | ||||||
| Surface negative regulators | ||||||
| Lag3& | Lymphocyte-activation gene 3 | NM_008479.1 | 1.7 | 5.2 | +210 % | 1.7 |
| Btla | B and T lymphocyte associated | NM_177584.3 | 1.8 | 3.2 | +80 % | 2 |
| Cd69 | Cluster of Differentiation 69 | XM_132882.1 | 3.9 | 5.4 | +39 % | 1.4 |
| IFN-dependent transcripts | ||||||
| Rsad2 | Radical S-adenosyl methionine domain containing 2 | NM_021384.2 | 4.9 | 9.5 | +94 % | 1.6 |
| Trim30 | Tripartite motif-containing 30 | NM_009099 | 2.2 | 3.1 | +40 % | 1.1 |
| Mx2 | Myxovirus (influenza virus) resistance 2 | NM_013606 | 6.0 | 8.2 | +37 % | 1.0 |
| Irf4 | Interferon regulatory factor 4 | NM_013674.1 | 4.5 | 5.3 | +18 % | 1.4 |
| Signaling elements | ||||||
| Gbp11 | Guanylate binding protein 11 | NM_01039647.1 | 3.2 | 7 | +120 % | 1.1 |
| Ppic | Peptidylprolyl isomerase C | NM_008908.1 | 1.8 | 3.2 | +80 % | 1.8 |
| Tyki | Thymidylate kinase family LPS-inducible member | NM_020557.3 | 4 | 5.7 | +42 % | 1 |
| Tagap | T-cell activation RhoGTPase activating protein | NM_145968.1 | 4.7 | 6.4 | +35 % | 1.4 |
| Tnfsf8 | Tumor necrosis factor (ligand) superfamily, member 8 | NM_009403.2 | 2.2 | 2.9 | +31 % | 1.1 |
| Dusp6 | Dual specificity phosphatase 6 | NM_026268.1 | 3.4 | 4.3 | +26 % | 1.4 |
| Gfod1 | Glucose-fructose oxidoreductase domain containing 1 | NM_001033399.1 | 6.2 | 7.8 | +26 % | 1.0 |
| Nfatc1& | Nuclear factor of activated T-cells, cytoplasmic 1 | NM_16791 | 3.3 | 4 | +21 % | 1.3 |
| Only CBD upregulated | ||||||
| Anti-oxidant and anti-inflammatory | ||||||
| Mt1 | Metallothionein 1 | NM_013602.2 | 0.5 | 2.3 | +330 % | 3 |
| Slc30a1 | Solute carrier family 30 (zinc transporter), member 1 | NM_009579 | 0.9 | 1.8 | +100 % | 1.8 |
| Mt2 | Metallothionein 2 | NM_008630.1 | 1 | 1.9 | +80 % | 2.3 |
| Negative regulators of T cell activity | ||||||
| Trat1 | T cell receptor associated transmembrane adaptor 1 | NM_198297 | 0.7 | 2.1 | +200 % | 1.6 |
| IFN-dependent transcripts | ||||||
| Ephx1 | Epoxide hydrolase 1 | NM_010145.2 | 0.7 | 1.8 | +170 % | 2.4 |
| Ets2 | V-ets avian erythroblastosis virus | NM_011809.2 | 1.3 | 2.4 | +80 % | 1.3 |
| Signaling elements | ||||||
| Ddr1 | Discoidin domain receptor tyrosine kinase 1 | NM_007584.1 | 1.1 | 3 | +170 % | 2.4 |
| Pitpnm2 | Phosphatidylinositol transfer protein, membrane-associated 2 | NM_011256.1 | 1.2 | 2.9 | +150 % | 2.6 |
| Gpnmb | Glycoprotein (transmembrane) nmb | NM_053110.2 | 0.4 | 0.9 | +90 % | 2.2 |
| Asb2 | Ankyrin repeat and SOCS box-containing protein 2 | NM_023049.1 | 0.29 | 0.52 | +79 % | 1.38 |
| Slc3a2 | Solute carrier family 3 member 2 | NM_008577.2 | 1.3 | 2.3 | +70 % | 1.5 |
$The results are presented as fold change vs control (without MOG35-55 or CBD). Numbers in italics indicate a significant effect of CBD (p < 0.005) in non-stimulated TMOG. &result reported previously [25]