Figure 7.
LA-1 fails to reduce fibrosis in livers of ANIT-exposed Fibγ390-396A mice. Fibγ390-396A mice were fed a diet containing 0.025% ANIT for 6 weeks. Mice were treated with vehicle (1.5 μL DMSO in 100 μL sterile PBS) or LA-1 twice daily (0.4 mg/kg per day, IP) in weeks 5 and 6. (A) Sirius red staining was quantified as described in “Methods.” Hepatic expression of mRNAs encoding the profibrogenic genes: (B) COL1A1, (C) TGFβ1, (D) ITGβ6, and (E) TGFβ2 were determined by real-time qPCR. (F) Serum ALT activity was determined as described in “Methods.” Data are expressed as mean + SEM; n = 7 mice per group. Veh, vehicle.