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Involucrin expression is downregulated in the skin of interleukin-4 (IL-4) transgenic (Tg) mice. The expression of involucrin was compared between wild-type (WT) mice and IL-4 Tg mice at both mRNA and protein levels. Mouse GAPDH was used as the internal control. Values are expressed as the mean ± SEM (n = 3). *P < 0.05 versus WT mice. For immunofluorescence microscopy, involucrin, red; nucleus, blue. Arrow denotes the dermal–epidermal border. Experiments were repeated three times.
