FIG. 4.

Examples of zebrafish embryo insulinoma xenografts. (A) Zebrafish embryo injected with CM-RED cells into the yolk sac occupy a major part of the yolk sac and show distant metastases, whereas (B) in the presence of anti-CD90 mAb embryos display only small tumor clumps in the yolk sac and no metastases. (C) Direct injection within the duct of Cuvier results in distant metastases, whereas (D) in the presence of anti-CD90 mAb less and smaller metastases are present especially at day 5 postinjection. (E) Statistical evaluation of embryos with distant metastases after injection of CM-RED cells into the yolk sac or duct of Cuvier at day 3 and 5 postinjection. Color images available online at www.liebertpub.com/scd