Table 2.
Comparison of the experimental conditions of trapping and measurements of semiquinone in the Qo site. n.s., not shown or not performed; SMP, submitochondrial particles; cyt., cytochrome; R. caps., Rhodobacter capsulatus; S. cerev., Saccharomyces cerevisiae.
| de Vries et al. [31] | Zhang et al. [33] | Cape et al. [34] | Vennam et al. [35] | Sarewicz et al. [36] |
|||
|---|---|---|---|---|---|---|---|
| SQo uncoupled | SQo–FeS spin-coupled | ||||||
| aerobic (A) or anaerobic (AN) | A | AN | AN | AN | A | A | |
| external oxidant | cyt. c | cyt. c2 | none | cyt. c | cyt. c | cyt. c | |
| isolated protein (I) or membranes (M) | M (SMP) | M | I | I | I | I | |
| source/organism | beef heart | R. caps. | R. caps. | R. caps. and S. cerev. | R. caps. | R. caps. | |
| temperature of detection (K) | CW EPR | 50 | 130 | 77 | 77 | 105–210 | 20 |
| pulsed EPR | n.s. | n.s. | 60 | 10–100 | n.s. | 10, 20 | |
| Qi site blocked by antimycin | yes | yes | yes | yes | yes | yes | |