Skip to main content
. Author manuscript; available in PMC: 2016 Jun 3.
Published in final edited form as: FEBS J. 2014 Apr 1;281(9):2190–2201. doi: 10.1111/febs.12773

Figure 1. Purification of MTSL-labelled 980C-ABCB1.

Figure 1

Fractions were collected at each stage of the IMAC and PD-10 based purification strategy. The figure corresponds to a 7.5% SDS-PAGE gel stained with PageBlue. FT refers to the flow through following binding stage and represents 0.05% of the total fraction volume. All wash fractions and the elution (E) represent 1% of the total volume, whilst the post PD-10 fraction (GF) corresponds to 0.05% of the volume. Mw refers to the molecular weight markers. All fractions were TCA precipitated prior to the electrophoresis.