Fig. 4.
DG lipase activity of rat DDHD2 expressed in CHO cells. (A) The DG lipase activity was measured using 9 µM [14C]SAG as a substrate. The protein samples used for the enzyme assay were the soluble (open circles) and the membrane (closed circles) fractions of CHO cells expressing HA-rDDHD2, and were the soluble (open squares) and the membrane (closed squares) fraction of vector-transfected CHO cells. (B) The soluble fraction (25 µg) and membrane fraction (25 µg) from the CHO cells were used for western blotting. An anti-rat DDHD2 antibody (upper panel) and an anti-HA antibody (lower panel) were used. Data are the means of triplicate measurements. Error bars represent standard error of the mean. Data are representative of three independent experiments. *P < 0.01 for the soluble fraction of CHO cells expressing HA-rDDHD2 versus the soluble fraction of vector-transfected CHO cells.