Fig. 7.

Effects of inhibitors on the partially purified rat brain DG lipase and HA-rDDHD2 expressed in CHO cells. DG lipase activity was measured in the presence of various concentrations of RHC80267 (closed circles for rat brain DG lipase, open circles for CHO-rDDHD2) and THL (closed squares for rat brain DG lipase, open squares for CHO-rDDHD2). The lipase inhibitors were added to the mixture before preincubation. Data are the means of triplicate measurements. Error bars represent standard error of the mean. Data are representative of three independent experiments.