Fig. 8.

pH dependency of the partially purified DG lipase. DG lipase activity in the active fractions (20 µg per assay) from the CHT 5-I column was measured at various pH levels. Buffers used for the experiments were MOPS-NaOH (closed triangles), Tris-HCl (open circles) and glycine-NaOH (closed circles). Except for the buffer used, assay conditions were the same as those for the standard assay. Data are the means of triplicate measurements. Error bars represent standard errors of the mean. Data are representative of three independent experiments.