Table I.

Subcellular localization of DG lipase from a rat brain

Fraction	Total protein (mg)	Specific activity (nmol/10 min/mg)	Total activity (nmol/10 min)
600 × g ppt	3.8	6.8	25.8
10,000 × g ppt	2.8	12.6	35.2
105,000 × g ppt	0.6	4.4	2.6
105,000 × g sup	18.4	2.1	38.6

DG lipase activity in subcellular fractions from rat brain was measured in the presence of 5 mM DOC as described in Materials and Methods. Enzyme activity was represented as the production of 2-AG in a 10-min reaction. Values are from one typical measurement among several similar experiments.