Table II.
Partial purification of DG lipase from rat brains
| Purification steps | Total protein (mg) | Total activity (nmol/ 10 min) | Specific activity (nmol/ 10 min/mg) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
| 105,000 × g sup | 534 | 909 | 1.7 | 100 | 1 |
| (NH4)2SO4 ppt | 285 | 685 | 2.4 | 75 | 1.4 |
| POROS HP2 | 138 | 649 | 4.7 | 71 | 2.8 |
| POROS HQ | 23 | 553 | 25 | 61 | 14 |
| CHT 5-I | 0.36 | 366 | 1,013 | 40 | 596 |
| Superdex 200 | 0.03 | 61 | 2,037 | 6.7 | 1,198 |
DG lipase was purified from 24 rat brains as described in Materials and Methods. Enzyme activity was represented as the production of 2-AG in a 10-min reaction. Values are from one typical measurement among several similar experiments.