Table III.
Proteins detected in the final purification sample by mass spectrometry
| Rank in MASCOT score | Annotation of protein | Hydrolase | This study |
|---|---|---|---|
| 1 | Dihydropyrimidinase-like 2 | Yes | Yes |
| 2 | rCG35703 | ||
| 3 | Neural cell adhesion molecule 1 | ||
| 4 | Microtubule-associated protein 1A | ||
| 5 | Ncam1 protein | ||
| 6 | Tenascin R | ||
| 7 | Neural cell adhesion molecule 1 | ||
| 8 | Acyl-peptide hydrolase | Yes | Yes |
| 9 | Similar to DDHD domain containing 2 | Yes | Yes |
| 10 | Poly(A) binding protein, cytoplasmic 1 | ||
| 11 | Gephyrin | ||
| 12 | TUC-4b | Yes | Yes |
| 13 | Deaminase 3 | Yes | |
| 14 | Dynactin 2 | ||
| 15 | Microtubule associated protein 1A | ||
| 16 | Adenosine monophosphate deaminase 2 (isoform L) | Yes | |
| 17 | Ubiquilin 2 | ||
| 18 | Similar to talin 2 isoform 2 | ||
| 19 | Poly(A) binding protein, cytoplasmic 3 | ||
| 20 | Glial fibrillary acidic protein | ||
| 21 | rCG31475 | ||
| 22 | Ceruloplasmin | ||
| 23 | Poly A binding protein, cytoplasmic 2 | ||
| 24 | Amylo-1,6-glucosidase | Yes | |
| 25 | Collapsin response mediator protein | Yes | |
| 26 | Beta actin | ||
| 27 | Spectrin, beta, non-erythrocytic 2 | ||
| 28 | Beta-adducin | ||
| 29 | Antidepressant-related protein ADRG123 | Yes | Yes |
| 30 | Similar to CG11448-PA |
The top 30 proteins in terms of the MASCOT score are listed. Nine proteins were regarded as hydrolases. Among them, five proteins were selected as candidate proteins for screening of DG lipase enzyme activity.