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. 2016 May 25;6(5):160075. doi: 10.1098/rsob.160075

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© 2016 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 6. — The percentage fraction of moving actin filaments versus α-actinin concentration for Zmys (black circles), Zmys-GFP (blue up triangles) and Zmys-HCRLC (red down triangles). Error bars indicate standard deviation for data from more than 1000 actin filaments at each α-actinin concentration and from three independent protein preparations. Lines extrapolated to the x-intercept indicate relative isometric force. Data at the highest α-actinin concentrations where linearity fails are excluded from the fits, because α-actinin is unable to fully inhibit actin filament sliding.