Table 1.

Zebrafish skeletal myosin actin-activated ATPase, in vitro motility and in vitro force.^a

	Zmys	Zmys-GFP	Zmys-HCRLC
Vmax (s−1)	16.7 ± 0.6	7.2 ± 0.8	—
KM (μM)	8.2 ± 1.4	42.1 ± 11.3	—
sm (µm s−1)b	1.39 ± 0.17	1.52 ± 0.16	—
isometric forcec (μg ml−1)	3.81 ± 0.42	2.50 ± 0.17	2.63 ± 0.22

^aV_max and K_M are defined in equation (2.1). All experimental errors indicated are standard deviation for 3 replicates (30–39 for s_m).

^bMotility velocity, s_m, is from the Qdot motility assay with myosin bulk concentration of 0.16 µM.

^cIn units of α-actinin concentration from data in figure 6.